Accurate, high-resolution cellular profiling — at the click of a button.

iSort™ is a robust, AI-powered digital cytometry platform for quantifying cell type composition and gene expression.

Accurate cell type proportions in minutes.

Starting with bulk gene expression data from blood or tissue samples, iSort™ Fractions provides quantified cell composition for each sample — at the click of a button. 



iSort™ boasts proven accuracy across sample types, library preparation kits, and RNA-seq methods, with even greater accuracy when paired with Agilent SureSelect targeted sequencing kits.

The first AI powered tool for inferring cell-type-specific gene expression.

Using grouped cell proportion data from iSort™ Fractions, iSort™ HiRes infers cell-type-specific gene expression.

Built on iSort’s powerful digital cytometry platform, HiRes provides an even deeper level of insight into immune cell makeup - and opens up a vast array of possibilities across life sciences research.

Expert-led Data Science Services tailored to your goals.

From consultation and planning to post analysis, reporting, and discussions — our end-to-end data science services are provided by a seasoned team of experts.

A collaborative approach ensures we support your goals whether that's custom signature matrices for tissues or species of interest, modeling for biomarker discovery, clinical outcome analysis, or other outcomes.

  • Start-to-end data processing - from data QC to analysis summary
  • Map and quantify RNA-seq reads, perform RNA-seq data QC
  • Run iSort™ Fractions
  • Run iSort™ HiRes
  • Create a custom signature matrix
  • Post data analysis tailored to your goals
A high throughput, low cost alternative for determining cell type composition.
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Throughput
Requires tissue dissocciation
Artifacts introduced by dissociation
Cost
Workflow simplicity
Manual data analysis
Number of cell types / cell type resolution

iSort™ Fractions

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No
No
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No
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Single-Cell
RNA Seq

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Yes
Yes
$$$$
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Yes
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Flow Cytometry /
CyTOF

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Yes
Yes
$$
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Yes
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IHC

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No
No
$$
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Yes
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A simple workflow backed by a powerful AI platform.

Starting from bulk tissue and blood gene expression data, iSort’s powerful AI-driven model quantifies cell-type specific composition and gene expression.

Sample & Library Prep

iSort™ is robust to sample and library preparation methods. No tissue dissociation required. Users may also start with an existing bulk RNA expression profile.

Bulk RNA Expression Profile

iSort™ is robust to RNA-seq platforms and alignment methods. 1,2

Run iSort™

iSort™ Fractions
Quantifies cellular composition from bulk expression data, using a predefined blood or tissue signature matrix.

iSort™ HiRes
Infers cell-type-specific gene expression from bulk expression data. Requires results from iSort™ Fractions.

Post Analysis

CiberMed can conduct post-analysis on the iSort™ output through data science services, or the client may analyze the output themselves.  

Boost accuracy with Agilent SureSelect technology.

Agilent SureSelect targeted sequencing panels — developed specifically for iSort™ Fractions blood and tissue modes — enable a combined SureSelect and iSort™ workflow for cellular profiling from bulk RNA-Seq data with enhanced sensitivity, accuracy, and robustness.

Our two flagship products — iSort™ Fractions and iSort™ HiRes — can be purchased as software as a service, or included as part of a collaborative data science services engagement tailored to support your goals.

FOOTNOTES
  1. Determining cell type abundance and expression from bulk tissues with digital cytometry, Newman, A. M. et al. Nature Biotechnology 37, 773-782 (2019)
  2. Additional data on file.